Universal and language-specific processing : the case of prosody

A key question in the science of language is how speech processing can be influenced by both language-universal and language-specific mechanisms (Cutler, Klein, & Levinson, 2005). My graduate research aimed to address this question by adopting a crosslanguage approach to compare languages with different phonological systems. Of all components of linguistic structure, prosody is often considered to be one of the most language-specific dimensions of speech. This can have significant implications for our understanding of language use, because much of speech processing is specifically tailored to the structure and requirements of the native language. However, it is still unclear whether prosody may also play a universal role across languages, and very little comparative attempts have been made to explore this possibility. In this thesis, I examined both the production and perception of prosodic cues to prominence and phrasing in native speakers of English and Mandarin Chinese. In focus production, our research revealed that English and Mandarin speakers were alike in how they used prosody to encode prominence, but there were also systematic language-specific differences in the exact degree to which they enhanced the different prosodic cues (Chapter 2). This, however, was not the case in focus perception, where English and Mandarin listeners were alike in the degree to which they used prosody to predict upcoming prominence, even though the precise cues in the preceding prosody could differ (Chapter 3). Further experiments examining prosodic focus prediction in the speech of different talkers have demonstrated functional cue equivalence in prosodic focus detection (Chapter 4). Likewise, our experiments have also revealed both crosslanguage similarities and differences in the production and perception of juncture cues (Chapter 5). Overall, prosodic processing is the result of a complex but subtle interplay of universal and language-specific structure

Juncture prosody across languages : similar production but dissimilar perception

How do speakers of languages with different intonation systems produce and perceive prosodic junctures in sentences with identical structural ambiguity? Native speakers of English and of Mandarin produced potentially ambiguous sentences with a prosodic juncture either earlier in the utterance (e.g., “He gave her # dog biscuits,” “他给她 # 狗饼干”), or later (e.g., “He gave her dog # biscuits,” “他给她狗 # 饼干”). These production data showed that prosodic disambiguation is realized very similarly in the two languages, despite some differences in the degree to which individual juncture cues (e.g., pausing) were favoured. In perception experiments with a new disambiguation task, requiring speeded responses to select the correct meaning for structurally ambiguous sentences, language differences in disambiguation response time appeared: Mandarin speakers correctly disambiguated sentences with earlier juncture faster than those with later juncture, while English speakers showed the reverse. Mandarin speakers also showed higher levels of accuracy in disambiguation compared to English speakers, indicating language-specific differences in the extent to which prosodic cues are used. However, Mandarin, but not English, speakers showed a decrease in accuracy when pausing cues were removed. Thus even with high similarity in both structural ambiguity and production cues, prosodic juncture perception across languages can differ

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase 1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age  6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score  652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc = 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N = 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in Asia and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Integrating Non-Native Speaker Identity in Semantic and Pragmatic Processing

Little research to date has examined how listeners integrate cues to non-native speaker identity in real time sentence processing. Here, we examine listeners’ interpretation of the semantic and socio-pragmatic content of utterances produced by either a foreign accented speaker or a native speaker. Overall, our findings suggest that processing speed was slower in the presence of foreign accents. However, the extra perceptual demands of processing unfamiliar accents did not translate into listeners’ accuracy rates, and in certain sentence contexts, non-native speakers were also more likely to elicit higher semantic or pragmatic interpretation accuracy. Our findings show that non-native speaker identity plays an important role in listeners’ sentence interpretations

Listeners evaluate native and non-native speakers differently (but not in the way you think)

Speaking in a foreign accent has often been thought to carry many disadvantages. Here we probe the social evaluation of foreign-accented vs. native speakers using spoken utterances that either obey or violate the pragmatic principle of Informativeness. We show that listeners form different impressions of native and non-native speakers with identical pragmatic behavior: specifically, in contexts where violations of Informativeness can be detrimental to or misleading for the listener, people rated underinformative speakers more negatively on trustworthiness and interpersonal appeal compared to informative speakers, but this tendency was mitigated in some cases for speakers with foreign accents. Furthermore, this mitigating effect was strongest for less proficient non-native speakers who were presumably not fully responsible for their linguistic choices. Contrary to previous studies, we also find no consistent global bias against non-native speakers. Thus the fact that non-native speakers have imperfect control of the linguistic signal affects pragmatic inferences and social evaluation in ways that can lead to surprising social benefits

Which button will I press? Preference for correctly ordered counting sequences in 18-month-olds

Correct counting respects the stable order principle whereby the count terms are recited in a fixed order every time. The 4 experiments reported here tested whether precounting infants recognize and prefer correct stable-ordered counting. The authors introduced a novel preference paradigm in which infants could freely press two buttons to activate videos of counting events. In the "correct" counting video, number words were always recited in the canonical order ("1, 2, 3, 4, 5, 6"). The "incorrect" counting video was identical except that the number words were recited in a random order (e.g., "5, 3, 1, 6, 4, 2"). In Experiment 1, 18-month-olds (n = 21), but not 15-month-olds (n = 24), significantly preferred to press the button that activated correct counting events. Experiment 2 revealed that English-learning 18-month-olds' (n = 21) preference for stable-ordered counting disappeared when the counting was done in Japanese. By contrast, Experiment 3 showed that multilingual 18-month-olds (n = 24) preferred correct stable-ordered counting in an unfamiliar foreign language. In Experiment 4, multilingual 18-month-olds (N = 21) showed no preference for stable-ordered alphabet sequences, ruling out some alternative explanations for the Experiment 3 results. Overall these findings are consistent with the idea that implicit recognition of the stable order principle of counting is acquired by 18 months of age, and that learning more than one language may accelerate infants' understanding of abstract counting principles. (PsycINFO Database Recor

Universals of listening : equivalent prosodic entrainment in tone and non-tone languages

In English and Dutch, listeners entrain to prosodic contours to predict where focus will fall in an utterance. Here, we ask whether this strategy is universally available, even in languages with very different phonological systems (e.g., tone versus non-tone languages). In a phoneme detection experiment, we examined whether prosodic entrainment also occurs in Mandarin Chinese, a tone language, where the use of various suprasegmental cues to lexical identity may take precedence over their use in salience. Consistent with the results from Germanic languages, response times were facilitated when preceding intonation predicted high stress on the target-bearing word, and the lexical tone of the target word (i.e., rising versus falling) did not affect the Mandarin listeners' response. Further, the extent to which prosodic entrainment was used to detect the target phoneme was the same in both English and Mandarin listeners. Nevertheless, native Mandarin speakers did not adopt an entrainment strategy when the sentences were presented in English, consistent with the suggestion that L2 listening may be strained by additional functional load from prosodic processing. These findings have implications for how universal and language-specific mechanisms interact in the perception of focus structure in everyday discourse

Asymmetric efficiency of juncture perception in L1 and L2

In two experiments, Mandarin listeners resolved potential syntactic ambiguities in spoken utterances in (a) their native language (L1) and (b) English which they had learned as a second language (L2). A new disambiguation task was used, requiring speeded responses to select the correct meaning for structurally ambiguous sentences. Importantly, the ambiguities used in the study are identical in Mandarin and in English, and production data show that prosodic disambiguation of this type of ambiguity is also realised very similarly in the two languages. The perceptual results here showed however that listeners’ response patterns differed for L1 and L2, although there was a significant increase in similarity between the two response patterns with increasing exposure to the L2. Thus identical ambiguity and comparable disambiguation patterns in L1 and L2 do not lead to immediate application of the appropriate L1 listening strategy to L2; instead, it appears that such a strategy may have to be learned anew for the L2

Cue equivalence in prosodic entrainment for focus detection

Using a phoneme detection task, the present series of experiments examines whether listeners can entrain to different combinations of prosodic cues to predict where focus will fall in an utterance. The stimuli were recorded by four female native speakers of Australian English who happened to have used different prosodic cues to produce sentences with prosodic focus: a combination of duration cues, mean and maximum F0, F0 range, and longer pre-target interval before the focused word onset, only mean F0 cues, only pre-target interval, and only duration cues. Results revealed that listeners can entrain in almost every condition except for where duration was the only reliable cue. Our findings suggest that listeners are flexible in the cues they use for focus processing